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A broad molecular framework of how neural stem cells are specified toward astrocyte fate during brain development has proven elusive. Here we perform comprehensive and integrated transcriptomic and epigenomic analyses to delineate gene regulatory programs that drive the developmental trajectory from mouse embryonic stem cells to astrocytes. We report molecularly distinct phases of astrogliogenesis that exhibit stage- and lineage-specific transcriptomic and epigenetic signatures with unique primed and active chromatin regions, thereby revealing regulatory elements and transcriptional programs underlying astrocyte generation and maturation. By searching for transcription factors that function at these elements, we identified NFIA and ATF3 as drivers of astrocyte differentiation from neural precursor cells while RUNX2 promotes astrocyte maturation.

These transcription factors facilitate stage-specific gene expression programs by switching the chromatin state of their target regulatory elements from primed to active. Altogether, these findings provide integrated insights into the genetic and epigenetic mechanisms steering the trajectory of astrogliogenesis.

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'Tunnelling within the Bunya Phyllite of Legacy Way, Brisbane' (PDF). 15th Australasian Tunnelling conference: 131–140. Archived from the original The tunnel forms part of Brisbane City Council's TransApex transport plan which, Notably, the Legacy Way project won the '2013 Major Tunnelling Project of the Year' award in the. Floor bores to act as guide rails for the jacking works;. Apr 10, 2018 The CLEM7, Go Between Bridge and Legacy Way are operated under individual franchise agreements with Brisbane City Council. Queensland Motorways network. Queensland Motorways network map (PDF, 2.84 MB) Aug 31, 2016 Tunnels or roads with tunnels: short-distance routes (2–7 km) that are aimed at avoiding congestion 6.7.

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